日循环高温对蛋鸡抗氧化能力及免疫机能的影响

doi:10.11843/j.issn.0366-6964.2017.06.009

Abstract

Abstract:

The aim of present experiment was to simulate the variation of ambient temperature in laying hens house in summer, evaluate the effects of different cyclic high temperature on antioxidant capacity and immune function of laying hens, and attract the attention of producers and researchers for high environmental temperature in summer. Two hundred eighty-eight Hy-line Brown hens, 28 weeks of age, were randomly divided into 4 groups and housed in 4 environment controlled chambers. Four groups were normal thermal group (NT, 21 ℃, ad libitum), low cyclic temperature group (LCT, 27-30 ℃, ad libitum), high cyclic temperature group(HCT, 29-35 ℃,ad libitum), and pair-feeding group (PF, 21 ℃, feeding amount was the same as that of high cyclic temperature group chickens), respectively. The experiment lasted for 4 weeks. There was 6 repeats in each treatment, and 12 chicken in each repeat. Core body temperature (TC) was recorded using a miniature temperature recorder during 3 days before the end of the experiment. At the end of the experiment, one hen from each repeat was randomly selected and blood samples were collected via the wing vein and serum was separated and stored at -20 ℃. The hens were then slaughtered, the liver and spleen were collected and stored at -20 ℃, the spleen index was determined. MDA, SOD, GSH-Px, CAT and T-AOC in serum and liver, IgA, IgG, IgM concentration in serum and lysozyme in serum were determined.The results showed that, compared with NT group, no significant differences were found in core body temperature in LCT group, and no significant differences were detected in MDA and antioxidant enzyme activity in serum and liver except the T-AOC concentration, which was significantly decreased in serum (P<0.05), and IgA and IgM concentration in serum were also significantly decreased in LCT group(P<0.05). While compared with NT group, the HCT group significantly increased TC and MDA concentration(P<0.05), decreased T-AOC concentration in serum and liver(P<0.05), the GSH-Px activity in serum also had a tendency to decline(0.05< P <0.1), IgA, IgM concentration in serum and spleen index were all significantly decreased in HCT group(P<0.05).Compared with the PF group,TC of HCT group was significantly increased(P<0.05), MDA concentration was significantly increased and T-AOC concentration was significantly decreased in serum and liver(P<0.05), GSH-Px activity in serum was also significantly decreased(P<0.05). Compared with the PF group, only IgA concentration in serum of HCT group was significantly decreased(P<0.05). The results of present study indicated that antioxidant capacity in serum and liver was not significantly affected, while IgA and IgM concentration in serum were significantly decreased in 27-30 ℃ cyclic temperature group. And 29-35 ℃ cyclic temperature group significantly increased TC, decreased antioxidant capacity in serum and liver, and decreased spleen index and IgA, IgM concentration in serum. The effect of cyclic temperature on antioxidant capacity and immune function may be associated with the rise of body temperature and the reduction of feed consumption, respectively.

CLC Number:

S831.4

DIAO Hua-jie, FENG Jing-hai, WANG Xue-jie, DIAO Xin-ping. Effect of Cyclic High Temperature on Antioxidant Capacity and Immune Function of Laying Hens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1044-1053.

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Effect of Cyclic High Temperature on Antioxidant Capacity and Immune Function of Laying Hens

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